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custom designed sybr plate  (Bio-Rad)


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    Bio-Rad custom designed sybr plate
    Custom Designed Sybr Plate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-designed+software/PrimePCR+Analysis+Software/pm40646626-121-64-67
    Average 99 stars, based on 217 article reviews
    custom designed sybr plate - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells
    Article Snippet: Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. .. The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a custom-designed SYBR plate (Bio-Rad), following the manufacturer’s protocol. ..

    Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells.
    Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a custom-designed SYBR plate (Bio-Rad), following the manufacturer’s protocol. ..

    Gene Expression:

    Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety
    Article Snippet: .. The gene expression study included cell line evaluation by custom-designed PrimePCRTM Assay plates (Bio-Rad) according to the instructions manual and regular qRT-PCR for genes presented in Supplementary Table 1. ..

    Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety.
    Article Snippet: .. The gene expression study included cell line evaluation by custom-designed PrimePCRTM Assay plates (Bio-Rad) according to the instructions manual and regular qRT-PCR for genes presented in Supplementary Table 1. ..

    Quantitative RT-PCR:

    Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety
    Article Snippet: .. The gene expression study included cell line evaluation by custom-designed PrimePCRTM Assay plates (Bio-Rad) according to the instructions manual and regular qRT-PCR for genes presented in Supplementary Table 1. ..

    Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety.
    Article Snippet: .. The gene expression study included cell line evaluation by custom-designed PrimePCRTM Assay plates (Bio-Rad) according to the instructions manual and regular qRT-PCR for genes presented in Supplementary Table 1. ..

    Reverse Transcription:

    Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells.
    Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a custom-designed SYBR plate (Bio-Rad), following the manufacturer’s protocol. ..

    Article Title: Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress
    Article Snippet: The eWAT denervation procedure was validated using male mice with a GFP reporter in CGRP+ sensory neurons,37,38 B6.129P2(Cg)Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc (Calca; stock number: 036773- UNC; citation ID: RRID:MMRRC_036773-UNC) as detailed in the Data Supplement. .. Frozen tissue (n=5–8 per group) was used to extract mRNA as reported previously.28 A custom-designed Real Time quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) array (Bio-Rad PrimePCR; Bio-Rad Laboratories, Inc) included the following targets: Tph1 (tryptophan hydroxylase 1), Htr2a (hydroxytryptamine [serotonin] receptor 2A), TrpV1, Ngf (nerve growth factor), Bdkrb1 (bradykinin receptor, beta 1), Bdkrb2 (bradykinin receptor, beta 2), NOX4 (NADPH oxidase 4), p47phox, Ilb1 (interleukin 1 beta), Tnf (tumor necrosis factor), Lepr (leptin receptor), Cybb (cytochrome b-245, beta polypeptide), Ptgs2 (prostaglandin-endoperoxide synthase 2), Cy2c44 (cytochrome P450-family 2, subfamily c-polypeptide 44), VEGFa (vascular endothelial growth factor A), Trpa1 (transient receptor potential cation channel, subfamily A, member 1), and IL17 (interleukin 17). ..

    cDNA Synthesis:

    Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells.
    Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a custom-designed SYBR plate (Bio-Rad), following the manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells.
    Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a custom-designed SYBR plate (Bio-Rad), following the manufacturer’s protocol. ..

    Article Title: CaMKII oxidation is a critical performance/disease trade-off acquired at the dawn of vertebrate evolution
    Article Snippet: For mast cells, 2.5 μg of the total RNA from each sample were converted into cDNA with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA catalog # 1708840). .. In all, 10 ng of cDNA were used in each qPCR reaction in custom-designed PrimePCR assay plates containing all target genes listed in Supplementary Data and three additional reference genes ( Hprt , Rps18 , and Ppia ) on a CFX384 Real-time PCR detection system (Bio-Rad, Hercules, CA). .. The primer validation data for primers on the assay plates are provided by Bio-Rad and are included in Supplementary Data . qPCR data were analyzed by the software Bio-Rad CFX Manager 3.1.

    Polymerase Chain Reaction:

    Article Title: Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress
    Article Snippet: The eWAT denervation procedure was validated using male mice with a GFP reporter in CGRP+ sensory neurons,37,38 B6.129P2(Cg)Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc (Calca; stock number: 036773- UNC; citation ID: RRID:MMRRC_036773-UNC) as detailed in the Data Supplement. .. Frozen tissue (n=5–8 per group) was used to extract mRNA as reported previously.28 A custom-designed Real Time quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) array (Bio-Rad PrimePCR; Bio-Rad Laboratories, Inc) included the following targets: Tph1 (tryptophan hydroxylase 1), Htr2a (hydroxytryptamine [serotonin] receptor 2A), TrpV1, Ngf (nerve growth factor), Bdkrb1 (bradykinin receptor, beta 1), Bdkrb2 (bradykinin receptor, beta 2), NOX4 (NADPH oxidase 4), p47phox, Ilb1 (interleukin 1 beta), Tnf (tumor necrosis factor), Lepr (leptin receptor), Cybb (cytochrome b-245, beta polypeptide), Ptgs2 (prostaglandin-endoperoxide synthase 2), Cy2c44 (cytochrome P450-family 2, subfamily c-polypeptide 44), VEGFa (vascular endothelial growth factor A), Trpa1 (transient receptor potential cation channel, subfamily A, member 1), and IL17 (interleukin 17). ..

    Article Title: Utility of clinical comprehensive genomic characterization for diagnostic categorization in patients presenting with hypocellular bone marrow failure syndromes
    Article Snippet: Droplet digital PCR (ddPCR) analysis was performed using the Bio-Rad Droplet Digital PCR system (BioRad, CA, USA) following manufacturer’s protocols. .. An allele-specific PCR assay to detect and quantify the fractional abundance of the RUNX1 c.496C>T variant and corresponding wildtype allele was custom designed using PrimePCR (BioRad). ..

    Variant Assay:

    Article Title: Utility of clinical comprehensive genomic characterization for diagnostic categorization in patients presenting with hypocellular bone marrow failure syndromes
    Article Snippet: Droplet digital PCR (ddPCR) analysis was performed using the Bio-Rad Droplet Digital PCR system (BioRad, CA, USA) following manufacturer’s protocols. .. An allele-specific PCR assay to detect and quantify the fractional abundance of the RUNX1 c.496C>T variant and corresponding wildtype allele was custom designed using PrimePCR (BioRad). ..



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    Image Search Results


    Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

    Journal: Brain Structure & Function

    Article Title: Temporal progression of pathological features in an α-synuclein overexpression model of Parkinson’s disease

    doi: 10.1007/s00429-025-02959-9

    Figure Lengend Snippet: Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

    Article Snippet: The detection of global activity, locomotion and mean velocity was fully automated using the custom-designed software Actitrack (Panlab, Spain).

    Techniques: Control, Injection, Activity Assay, Software