custom designed sybr plate (Bio-Rad)
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Custom Designed Sybr Plate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-designed+software/PrimePCR+Analysis+Software/pm40646626-121-64-67
Average 99 stars, based on 217 article reviews
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Expressing:Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells Article Snippet: Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. .. The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells. Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a Gene Expression:Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety Article Snippet: .. The gene expression study included cell line evaluation by Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety. Article Snippet: .. The gene expression study included cell line evaluation by Quantitative RT-PCR:Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety Article Snippet: .. The gene expression study included cell line evaluation by Article Title: Development of a novel gene expression panel for the characterization of MSCs for increased biological safety. Article Snippet: .. The gene expression study included cell line evaluation by Reverse Transcription:Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells. Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a Article Title: Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress Article Snippet: The eWAT denervation procedure was validated using male mice with a GFP reporter in CGRP+ sensory neurons,37,38 B6.129P2(Cg)Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc (Calca; stock number: 036773- UNC; citation ID: RRID:MMRRC_036773-UNC) as detailed in the Data Supplement. .. Frozen tissue (n=5–8 per group) was used to extract mRNA as reported previously.28 A custom-designed cDNA Synthesis:Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells. Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a Real-time Polymerase Chain Reaction:Article Title: Exposure to polystyrene nanoparticles leads to dysfunction in DNA repair mechanisms in Caco-2 cells. Article Snippet: .. Each sample (1 μg RNA) was subjected to reverse transcription using the iScript cDNA Synthesis Kit from Bio-Rad, employing RNase H + MMLV reverse transcriptase in a final reaction volume of 20 μl, as specified in the kit’s instructions. qPCR assay The expression of genes was assessed in two Caco-2 cell lines (untreated vs. nanoparticle PNPs-100 μg/mL treated) using a PrimePCR assay with a Article Title: CaMKII oxidation is a critical performance/disease trade-off acquired at the dawn of vertebrate evolution Article Snippet: For mast cells, 2.5 μg of the total RNA from each sample were converted into cDNA with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA catalog # 1708840). .. In all, 10 ng of cDNA were used in each qPCR reaction in Polymerase Chain Reaction:Article Title: Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress Article Snippet: The eWAT denervation procedure was validated using male mice with a GFP reporter in CGRP+ sensory neurons,37,38 B6.129P2(Cg)Calcatm1.1(EGFP/HBEGF)Mjz/Mmnc (Calca; stock number: 036773- UNC; citation ID: RRID:MMRRC_036773-UNC) as detailed in the Data Supplement. .. Frozen tissue (n=5–8 per group) was used to extract mRNA as reported previously.28 A custom-designed Article Title: Utility of clinical comprehensive genomic characterization for diagnostic categorization in patients presenting with hypocellular bone marrow failure syndromes Article Snippet: Droplet digital PCR (ddPCR) analysis was performed using the Bio-Rad Droplet Digital PCR system (BioRad, CA, USA) following manufacturer’s protocols. .. An allele-specific PCR assay to detect and quantify the fractional abundance of the RUNX1 c.496C>T variant and corresponding wildtype allele was custom designed using Variant Assay:Article Title: Utility of clinical comprehensive genomic characterization for diagnostic categorization in patients presenting with hypocellular bone marrow failure syndromes Article Snippet: Droplet digital PCR (ddPCR) analysis was performed using the Bio-Rad Droplet Digital PCR system (BioRad, CA, USA) following manufacturer’s protocols. .. An allele-specific PCR assay to detect and quantify the fractional abundance of the RUNX1 c.496C>T variant and corresponding wildtype allele was custom designed using |
